"Ninety per cent of us in the armed forces are really unhappy," said Colonel Ruben Paz Jimenez said in a video released on Saturday. "We are being used to keep them in power."
He urged his fellow soldiers to help allow humanitarian aid into Venezuela. The shipment of US aid is currently in Cucuta, Colombia, on the border.
Maduro has vowed to prevent the aid from entering, labeling it a precursor of a US invasion.
A week ago, Air Force General Francisco Yanez also dropped his allegiance to Maduro. The military's backing is critical to the sway of power in Venezuela.
Venezuela's Guaido won't rule out US intervention to force Maduro's hand
Venezuela's self-proclaimed acting president Guaido on Friday refused to rule out the possibility of authorising United States intervention to help force President Nicolas Maduro from power and alleviate a humanitarian crisis.
The opposition leader launched a bid to oust Maduro last month, declaring himself interim president, a move recognised by the US and around 50 other countries, including 20 from the European Union.
Under Maduro's stewardship, oil-rich Venezuela's economy has collapsed leaving the country wracked by hyperinflation, recession and shortages of basic necessities such as food and medicine.
COMMENTS
Comments are moderated and generally will be posted if they are on-topic and not abusive.
For more information, please see our Comments FAQ