The train was travelling at 140 kilometres an hour (87 mph) between Nimes and Montpellier when it hit a tree that had been uprooted by a hailstorm, they said.
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Some of the injured were in an "absolutely urgent" state and another 50 people suffered minor injuries.
France has shut around 20 mosques since December
"At Lunel at around 3:45 pm, a TER (regional train) going at 140 kmh hit a tree which had fallen on to the track after extremely violent storms in the region," national rail operator SNCF said.
"Firefighters and police are at the scene where access is very difficult," SNCF added.
Accident #TER, bilan officiel des secouristes : 3 blessés graves, 10 blessés légers.
— Groupe SNCF (@GroupeSNCF) August 17, 2016
The rail company did not give any information about how many passengers had been on the train at the time of the accident.
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