Broad, who underwent a scan on his right foot after Friday's play in the ongoing second Test, has suffered damage to a tendon.
Kohli fifty after Ashwin's fifer puts India in driver's seat against England
"Stuart Broad has strained a tendon and one of the small joints in his right forefoot. The injury will be reviewed further at the end of the Test," an England media statement said Saturday.
However Broad, who rattled the Indian top-order with his twin strikes in the final session on day three, will "continue to bowl" in the rest of the innings.
Broad, 30, also got Indian opener Lokesh Rahul for a duck in the second over of the match but struggled as the game progressed.
Kohli, Pujara tons give India opening day honours against England
Broad's injury scare has added to the woes of a team already missing leading paceman Chris Woakes who is nursing a knee injury.
Woakes' replacement James Anderson has just returned to action after recovering from a shoulder injury.
England have Steven Finn and Jake Ball in the squad as pace backup for the marathon five-match series. The first Test at Rajkot ended in a draw.
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