Cockpit recordings recovered from the crash site indicated one of the seats was pushed back and the door opened and closed. Then knocking is heard, said the source, adding "there was no more conversation from that point until the crash".
The source said an alarm indicating the proximity to the ground could be heard before the moment of impact.
All those on board were killed when Germanwings flight 4U 9525 from Barcelona to Duesseldorf crashed in remote terrain Tuesday, after an unexplained eight-minute descent in mid-flight.
No distress signal was sent from the Airbus A320 and the crew failed to respond to ground control's desperate attempts to make contact.
The cockpit recording showed the pilots speaking normally in German at the start of the flight, the source said, adding that it could not be determined if it was the captain or the first officer who left the cockpit.
The New York Times also cited a senior military official involved in the investigation as saying the black box data indicated one pilot was locked out of the cockpit and tried unsuccessfully to bash his way back in.
"The guy outside is knocking lightly on the door, and there is no answer," the investigator told the newspaper. "And then he hits the door stronger, and no answer. There is never an answer."
He continued: "You can hear he is trying to smash the door down."
Photos of the mangled black box retrieved at the site showed its metal casing torn and twisted by the violence of the impact. The casing of a second black box, the flight data recorder, has been found but not the device itself.
Investigators have said the plane was still flying when it smashed into the remote mountainside, with the force of the impact leaving only small pieces of debris scattered over a wide area.
A mountain guide who got near the crash site said he was unable to make out recognisable body parts.
"It's incredible. An Airbus is enormous. When you arrive and there's nothing there... it's very shocking," said the guide, who did not wish to be identified.
The crash site, which is situated at about 1,500 metres altitude, is accessible only by helicopter or an arduous hike on foot.
Arrangements were being made for the families of the victims, at least 51 of whom were Spaniards and at least 72 Germans, to gather near the crash site Thursday.
Lufthansa, which owns popular low-cost operator Germanwings, announced it would lay on two flights to take the victims' loved ones from Barcelona and Duesseldorf to the southern French city of Marseille.
The mountain village of Seyne-les-Alpes, which is serving as the base of the rescue operation, lies about 180 kilometres northeast of Marseille.
The families are not expected to travel as far as the crash site.
Helicopters began winching the remains of victims, found scattered across the scree-covered slopes, to Seyne-les-Alpes Wednesday, a source close to the investigation told AFP.
Lyon-based international police agency Interpol said it had dispatched a team of victim identification experts to the site.
Authorities on the ground who had the gruesome task of sifting through the debris called off the search at nightfall Wednesday, to be resumed at dawn Thursday.
Earlier Wednesday, the head of France's Bureau d'Enquetes et d'Analyses (BEA) crash investigation agency head Remi Jouty told reporters he still had "not the slightest explanation" for the tragedy at this stage.
"It is inexplicable," Lufthansa chief executive Carsten Spohr said in Frankfurt.
"The plane was in perfect condition and the two pilots were experienced."
Weather did not appear to be a factor in the crash, with conditions calm at the time, and Germanwings had an unblemished safety record.
French President Francois Hollande and German Chancellor Angela Merkel flew over the site to see the devastation for themselves Wednesday. Spain's Prime Minister Mariano Rajoy also visited a crisis centre near the scene.
"My deepest sympathies with the families and all my thanks for the friendship of the people of this region and in France," Merkel wrote in a book of condolence.
"We have to understand what happened. We owe it to the families and the countries concerned by the drama," Hollande told reporters.
The plane was carrying six crew and 144 passengers, including 16 German teenagers returning home from a school trip.
Bereaved pupils from their high school in the small German town of Haltern wept and hugged near a makeshift memorial of candles as they pulled together to share the pain of losing their friends.
"Yesterday we were many, today we are alone," read a hand-painted sign at the school, decorated with 16 crosses - one for each of the victims, most around 15 years old.
Compounding the tragedy, it emerged that those killed had won the trip in a lottery of their classmates, reported local daily the Halterner Zeitung.
Also on board were opera singers Oleg Bryjak, 54, and Maria Radner, 33, flying to their home city of Duesseldorf. Radner was travelling with her husband and baby, one of two infants on board the plane.
It was the deadliest air crash on the French mainland since 1974 when a Turkish Airlines plane crashed, killing 346 people.
Lufthansa said the aircraft was carrying citizens of 18 countries. Three Americans and three Britons were confirmed among the victims.
Argentina, Australia, Belgium, Colombia, Denmark, Holland, Israel, Japan, Mexico and Morocco also had nationals on board, according to officials.
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