Scientists create human eggs from skin cells

PARIS:

Scientists said Wednesday they have turned human skin cells into eggs and fertilised them with sperm in the lab for the first time — a breakthrough that is hoped to one day let infertile people have children.

The technology is still years away from potentially becoming available to aspiring parents, the US-led team of scientists warned. But outside experts said the proof-of-concept research could eventually change the meaning of infertility, which affects one in six people worldwide.

If successful, the technology called in-vitro gametogenesis (IVG) would allow older women or women who lack eggs for other reasons to genetically reproduce, Paula Amato, the co-author of a new study announcing the achievement, told AFP. "It also would allow same-sex couples to have a child genetically related to both partners," said Amato, a researcher at the Oregon Health & Science University in the United States.

Scientists have been making significant advances in this field in recent years, with Japanese researchers announcing in July they had created mice with two biological fathers.

But the new study, published in the journal Nature Communications, marks a major advance by using DNA from humans, rather than mice.

The scientists first removed the nucleus from normal skin cells and transferred them into a donor egg which had its nucleus removed. This technique, called somatic cell nuclear transfer, was used to clone Dolly the sheep in 1996.

However a problem still had to be overcome: skin cells have 46 chromosomes, but eggs have 23.

The scientists managed to remove these extra chromosomes using a process they are calling "mitomeiosis", which mimics how cells normally divide.

They created 82 developing eggs called oocytes, which were then fertilised by sperm via in vitro fertilisation (IVF).

After six days, less than nine percent of the embryos developed to the point that they could hypothetically be transferred to the uterus for a standard IVF process.

However the embryos displayed a range of abnormalities, and the experiment was ended.

While the nine-percent rate was low, the researchers noted that during natural reproduction only around a third of embryos make it to the IVF-ready "blastocyst" stage.

Amato estimated the technology was at least a decade away from becoming widely available.

"The biggest hurdle is trying to achieve genetically normal eggs with the correct number and complement of chromosomes," she said