Columbia University disciplined dozens of pro-Palestinian student protesters who seized part of the school's main library during a demonstration in early May that led to multiple arrests.
Following the protest, the university began an investigation into rules violations, banned participants from the campus and placed them on interim suspension, it said.
It issued its final determinations on Tuesday.
Columbia for Palestine, a pro-Palestinian group at the school, said on Tuesday that 80 students were informed on Monday about their punishments.
We support free speech. But demonstrations and other protest activities that occur inside academic buildings and places where academic activities take place present a direct impediment to maintaining our core academic mission.
Columbia UniversityIt said the disciplinary action marked "the most suspensions for a single political protest in Columbia campus history" and exceeded past disciplinary actions announced against people over other protests.
In March, the Trump administration said it was penalizing the university over how it handled last year's pro-Palestinian protests by canceling hundreds of millions of dollars in research grants.
It contended that Columbia's response to alleged antisemitism and harassment of Jewish and Israeli members of the university community was insufficient.
After the government announced the funding cancellations, the school announced a series of commitments in response to the administration's concerns.
Read: Columbia University calls NYPD as pro-Palestinian protests escalate
"We support free speech," the school said in a statement at the time. "But demonstrations and other protest activities that occur inside academic buildings and places where academic activities take place present a direct impediment to maintaining our core academic mission."
Protesters, including some Jewish groups, say the Trump administration has wrongly conflated their criticism of Israel's military assault in Gaza with antisemitism and their advocacy for Palestinian rights with support for extremism.
Israel's devastating military campaign in Gaza followed a deadly October 2023 attack on Israel by Hamas, the militant organization that governed Gaza.
Read More: US professors take Trump's immigration policies to court over pro-Palestinian activities
Last week, Columbia adopted a controversial definition of antisemitism that equates it with opposition to Zionism.
The school also said it would no longer engage with pro-Palestinian group Columbia University Apartheid Divest.
The government has attempted to use the leverage of federal funding with other educational institutions as well, including Harvard University, over campus protests. It has also tried deporting some foreign pro-Palestinian students but has faced judicial roadblocks.
Rights advocates have raised concerns about due process, academic freedom and free speech over the government's actions.
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