He said this was "concluded" during Wednesday's visit of US Secretary of State Rex Tillerson to Moscow.
But in Washington, the US State Department said Tillerson did not eliminate the possibility the United States may undertake future strikes.
Putin says US-Russia ties worse since Trump took office
"The secretary explained there were no subsequent targets after the missile strike, but he did not rule out any future action," State Department acting spokesperson Mark Toner said in a statement.
"He stressed that Russia is in a position to use its influence over the Assad regime to ensure it is never again necessary for the US to act," Toner said.
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