San Francisco Superior Court Judge Anne-Christine Massullo based her decision on an agreement Mimi Lee and Stephen Findley signed at a fertility clinic in 2010, when Lee was diagnosed with cancer.
The document stipulated that five embryos the couple decided to cryogenically freeze to preserve their chance to have children would be thawed and destroyed in the event of divorce.
Trial over fate of frozen embryos underway in San Francisco
"It is a disturbing consequence of modern biological technology that the fate of the nascent human life, which the embryos in this case represent, must be determined in a court by reference to cold legal principles," the judge wrote.
The case has been watched with interest in the United States as a test of the fate of frozen embryos in divorce cases.
Lee, a 46-year-old pianist and part-time anesthesiologist, unsuccessfully argued that the embryos were her last chance at having biological children because she was left infertile following treatment for breast cancer.
Her ex-husband, an investment executive, however insisted that the consent agreement the couple signed was clear and legally binding.
The couple have been embroiled in a bitter legal battle over the embryos since their divorce in 2013. The judge's ruling can be appealed.
Attorneys representing Lee and Findley could not be immediately reached for comment.
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