Earlier, Younus was controversially included in the ODI team, after his consistent run of good performances in the longest format of the game.
Younus Khan set to call it quits in One-Day cricket: report
The 37-year-old had wanted to leave the format on a high after he was selected in the 16-man squad for the four-match series against England despite patchy form.
Going into his last match, Younis has scored 7,240 runs with seven hundreds and 48 half-centuries in one dayers.
"He will retire from one-day cricket after Wednesday's game and he has conveyed this to us," team manager Intikhab Alam told AFP.
He was dropped from the ODI team after poor performances in the 2015 World Cup, in which he only managed to score 37 runs in three matches, with an average of just 14.33 with the bat.
Younus made his debut back in 2000 against Sri Lanka at National Stadium Karachi, and have so far represented Pakistan in 264 ODI’s, scoring 7,240 runs with an average of 31.34, with a highest of 144 against Hong Kong in 2004.
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He managed to score seven hundreds and 48 fifties in his career that lasted over 15 years. He also captained Pakistan in One-Day cricket from 2009-10 and took Pakistan to the semi-finals of the Champions Trophy in 2009, which held in South Africa.
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