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There were no bees on the plane in the Wednesday incident, which was spotted by a service crew who saw the bees buzzing under the wing.
The airline called in a local beekeeper to handle the bees so the plane from Las Vegas could turn around as a flight to Frankfort, Germany.
"Unfortunately, not long after the beekeeper left, another swarm decided to visit the same aircraft," American Airlines spokeswoman Andrea Huguely said.
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The beekeeper was called again to remove the second swarm of bees. No one was stung. "This was not our first encounter with bees," Huguely said.
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