The Chief Judicial Magistrate's Court in Dhaka formally accepted a charge sheet from police against Sohel Rana, 35, and the others in connection with the collapse, the country's worst ever industrial disaster.
"We've charged 41 people including the owner of the building, Sohel Rana, with murder over the collapse of Rana Plaza in April 2013," lead investigator Bijoy Krishna Kar told AFP, addind that all face the death penalty if convicted.
Read: Owner of collapsed Bangladesh factory to face murder charges
Among the others charged are seven owners of the individual factories housed in the complex and 12 government officials whose responsibilities included safety and inspections of the building.
"It is the biggest industrial disaster in Bangladesh's history. And all 41 of them have collective responsibility for this mass killing of more than 1,100 innocent people," Kar said.
Rana became Bangladesh's public enemy number one after survivors recounted how thousands of them were forced to enter the compound at the start of the working day despite complaints about cracks appearing in the walls.
Rana was arrested on the western border with India as he tried to flee the country, in the days after the April 24 disaster.
Rana and 17 others have also been charged with violating the building code, for extending the six-storey structure -- which was initially approved as a shopping centre -- into a nine-storey factory complex.
"That illegal extension violating all construction (regulations) was the seed of this massive disaster," Kar said on Sunday.
The disaster highlighted appalling safety problems in Bangladesh's $25 billion garment industry, the world's second largest after China's.
A host of Western retailers had clothing made at Rana Plaza, including Italy's Benetton, Spain's Mango and the British low-cost chain Primark.
The disaster prompted sweeping reforms including new safety inspections and higher wages in the industry, which employs around four million workers.
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