"I hope that common sense will prevail, …that we will work together normally and that we and our partners will not cause harm by poking at one another," Putin was quoted as saying by Russian news agencies during a visit to the eastern Siberian city of Yakutsk.
The remarks came a day after European Union leaders gave Russia a week to reverse course in Ukraine or face a new round of sanctions.
Fears are growing that the confrontation could engulf the whole continent after Russia allegedly sent troops to back a new offensive by pro-Kremlin rebels in southeastern Ukraine.
Russian officials have said previously that they were preparing countermeasures in case of new Western sanctions, after having last month banned most EU and US food imports.
But Foreign Minister Sergei Lavrov earlier Monday said that Russia would not "slam the door shut" neither on Western trade nor exit from the World Trade Organisation (WHO).
"We will, above all, proceed from our own interests, defend our economy, defend our social system, defend our businesses..." Lavrov told Moscow university students.
Sanctions threats and reports of Russian tanks being involved in fighting outside of Lugansk sent the ruble to a new record low of 37.4527 in late morning trading.
It also sank to a five-month low of 49.1795 to the euro.
Russian stocks were also lower in early afternoon trading, with the ruble-denominated MICEX index shedding 0.33 percent and the dollar-denominated RTS slumping 1.30 percent after sharper falls on Friday.
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@unbelievable: By the same logic the US and partners should consider they might be over their heads in the Mid-east.
The US tried to get Russia involved with the Georgia adventure, and that did not workout very well. Now the US is trying its best to get a war going in the Ukraine, ably supported by its lackeys in the EU, together with other puppets, in the sanction war.. So far Russia has been the one voice of sanity and has shown admirable restraint, but the EU should not push it. Winter is coming on and the Europeans could get very cold if the gas supply is cut off, not to mention the economic disaster which could eventuate if sanctions go far enough. Unfortunately, whilst the people pushing the buttons in the various European capitals will stay warm the same will not happen to the little people, particularly those in the Ukraine attempting to live in houses which have been shelled to pieces by the junta in Kiev. Kiev is of course being fully funded by the West.
Speaking of "common sense" - how rational is it to send Russian troops into a neighboring country knowing you risk alienating your largest export customers? Chest thumping may get you a boost in popularity polls but the long term consequences of invasion may be more than Putin bargained for.