Pakistan went down by 117 runs in the fifth and final match in Sharjah on Monday, losing the series 4-1.
Batting was once again the problem with Pakistan, chasing a target of 269, folding for 151 in 35.3 overs.
Misbah said the consistent batting failures are worrying.
"As a captain this is very difficult for me and these are worrying signs for me before the World Cup 2015," he said after the match.
Pakistan have been let down by their batsmen since their 3-0 Test rout in South Africa. They also lost the one-day series 3-2 in February-March this year.
In June they lost all three of their matches in the Champions Trophy in England, and despite their one-day win in the West Indies the batting remains a headache.
In September Pakistan lost a one-day game and a Test match against cricketing minnows Zimbabwe.
"Every player has got adequate chances, more than two series, and yet they are not performing and it's a big worry," the captain said.
"But rather making a big hue and cry over it we must sit down and think seriously about it."
"As captain you are responsible," said Misbah, who took over as Test captain in 2010 and one-day skipper in 2011.
Asked if he wants to keep the captaincy until the 2015 World Cup, he replied: "I always say this and I maintain that I want to play as long as I am fit."
Misbah's counterpart AB de Villier said victory in the one-day series is a step in the right direction before the World Cup, to be jointly hosted by Australia and New Zealand.
The captain, who hit an unbeaten 115 on Monday, said the victory had lifted the Proteas after their 4-1 loss in Sri Lanka in July.
"We really hit an all-time low in Sri Lanka and moving up from there is pleasing to me and the coaching staff and the whole team," he said.
Batting was tough on a slow Sharjah stadium pitch.
"It was one of the harder innings that I have played up until 60-70 runs. It was bloody difficult," he said.
"Facing (Mohammad) Irfan there -- he was beating the bat -- was difficult. Saeed Ajmal was bowling well and (Shahid) Afridi bowled an amazing spell and I felt I couldn't get the ball off the square.
"But once I hit a couple in the middle in the 42 and 43rd overs it felt really good and I took my opportunity and followed through nicely," De Villiers said of his 102-ball knock studded with ten boundaries and three sixes.
The teams now play two Twenty20s in Dubai on Wednesday and Friday.
COMMENTS (11)
Comments are moderated and generally will be posted if they are on-topic and not abusive.
For more information, please see our Comments FAQ
@Singh: Boom Boom can still single handedly BUST the entire Indian team open!!!! GFYS!!
Misbah has at least curtailed chances of two deserving players in respective formats over the years. One is Hammad Azam in test & ODI format and other is experienced but unfortunate Faisal Iqbal test format. On one instant Ayub Dogar was preferred over Faisal in Sri Lanka. I think Misbah-Hafeez nexus is destroying Pakistan cricket.
Boom Boom bust like old tire.
We need Shoaib Malik as captain
I'm not a Misbah Basher (just for fun) .. but its either this man who's destroyed Pakistan cricket.. or the one who designed the strategy of 'utterly ridiculous defense'. Asad Shafiq, remember his very first 4 deliveries that he faced when he came into international cricket... All of them were beautifully timed 4's .. well placed.. wonderfully executed. Now he's an unstable twig trying to bounce back balls. Same is the story of Nasir Jamshed and many others. And there's only one person who had this disease before these players entered the arena... yes you guessed it right.
@M: 100% agreed. Misbah's low morale and defensive strategy is affecting Pakistan cricket, coupled with dreadful decision by patron in chief for appointing sethy.
Pakistan cricket is a lost case now. Pakistan should invest in some other game.