The shipment of Australian sheep was sent to the port city after being turned away by Bahrain, with Pakistan livestock officials ordering them to be culled over disease concerns.
Graphic footage of their slaughter was aired by ABC's Four Corners programme on Monday, including images of a man sawing at a sheep's neck before throwing it into a bloody trench.
Other sheep were bulldozed into the pit after being killed last month, but some were seen the next morning still breathing, sparking angry condemnation of their treatment.
Gillard said that she had spoken to Prime Minister Raja Pervaiz Ashraf at a summit of European and Asian leaders in Laos to express her concern.
"I did raise with the prime minister of Pakistan my concern about the graphic and very cruel images we've seen of the treatment of Australian sheep," she told reporters in Vientiane.
"I explained to him that Australians are distressed to see these acts of cruelty and that I wanted the matter investigated,” she said.
"He undertook to investigate the matter ... I was very clear about Australia's concerns, very strong in raising those concerns and very clear that this is something that has distressed the Australian people."
Australia Agriculture Minister Joe Ludwig earlier called the slaughter "appalling" and his department disputed that the sheep were unhealthy.
"We do not know the reasoning behind the Pakistan authority's decision to cull the sheep in Pakistan or their choice of the method used," the agriculture ministry said.
"We continue to hold that both the decision and the method used were unnecessary."
Livestock officials ordered the sheep to be culled after they tested positive for salmonella and actinomyces bacteria.
Although samples from the sheep were sent to a British laboratory and came back clean, clearing the meat for human consumption, municipal officials in Karachi rejected the tests.
Australian farmers urged against any consequent ban on the live export trade, saying significant improvements had been made in regulating Australia's live export market since a controversy in Indonesia last year.
Canberra suspended live cattle exports to Indonesia for a month last year after a television documentary revealed mistreatment inside its abattoirs, only reinstating the trade under a strict new licensing system.
Australia's National Farmers Federation said "decisive action" had been taken to temporarily suspend exports of sheep to Pakistan and Bahrain while investigations were carried out.
The farming lobby group stressed that Australia was a world leader in animal treatment in exports and warned that banning the trade would see welfare standards fall.
"If Australia was to stop exporting livestock, global animal welfare standards would unquestionably decline," it said.
Australia's live export trade is worth about US$1 billion a year and employs thousands of people.
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The Australian has reported that "Exporter Wellard yesterday conceded for the first time that "in hindsight" some of its decisions might have "contributed to the cull" in Pakistan. This included not telling Pakistan the sheep had already been rejected by Bahrain. Wellard maintains however the rejection was not the reason why local authorities triggered the cull."
It is very interesting to note that the Australians are almost exclusively focusing on issues of humane treatment of those sheep, much more than on their being tested positive by livestock officials for the salmonella and actinomyces bacteria.
If the Australians are so concerned about their sheep they are most welcome to take them back and feed them to their own people and refund. If they don't like this culling, they shouldn't export diseased animals in the first place! Raja Pervez Ashraf should have invited Julia Gillard to a lavish dinner where her entire delegation would be fed with those sheep.
It is very strange that White people would be so appalled by the alleged brutal treatment of farm animals but cheer worse brutalities committed by their own governments and armies against human beings of other races during savage wars of aggresstion aimed at grabbing their natural resources as well as during proxy terrorism sponsored by rulers they vote into office.
@asad, @Faisal, agree with you. This was horrific and disgusting barbarity and shows how low we have fallen as a country. Mullahs are too busy spreading hate to have time to condemn this slaughter. People should beware of the "budd duas" of innocent people and innocent animals.
This horrific slaughter should be condemned by the Islamic scholars
Pakistanis do this to their citizens so really no shock how they would treat the poor sheeps.
Why Bahrain rejected? There must be a reason. Why Pakistani business man accepted? Where they trying to make big profit after bribing the officials? Cruelty to animals is not acceptable in any manner.Why Pakistani media did not report this cruelty?
I saw the documentary yesterday ! it was disgusting ! the way the sheep had been killed ! they were still breathing the next day in a pit full of half alive half dead sheep lying in blood. We ( Pakistanis ) as a nation have become inhumane . If you listen to the voices in the videos they are commenting about taking out 1000-1200 sheep to sell them in the market. And this culling didnt affect Australian business in anyway ! what it did affect is Pakistan's image ! and that is further down the drain ! just an other international display of how stupid the nation is !
@Riaz Khan
Self hate much? Don't animal get slaughtered everywhere? Its ok to eat meat just don't show us? What nonsense. If aus are so sensitive they should all become vegetarian just don't tell us what to do. Stop sending defective products in 1st place. This is not 1st incident of australian defective product.
Australian PM forgot that she was talking to the PM of the nation for religion which celebrates in public the blood and violence...Just look at the videos of recent barbaric sacrificing tribal rituals that were performed few days back...Treatment to sheep was much more humane in comparison...But these people don't get it...I am sure the Pakistani PM must have thought after she left that I still don't get what was she talking about...
Amazing Julia Gillard...she does not get distressed a the treatment of refugees in Australia which despite being a nation of immigrant themselves they allow boats to capsized and let distressed immigrants drown and who put the rest in camps to suffer inhumanly. Hypocrites
Yeah, commenting people, just go right ahead and ignore that they ignored a test saying they were fine. Also, make sure to forget a large part of the issue is the way they went about culling them. Using a saw to decapitate them? That's just brutal and something you'd only expect from a psychopath in his youth.
@Azhar: Do you really know the whole story of what and when happened?
What if the Sheep were just fine and enough bribes were not paid to the concerned officials ...It been known to have happened before ..
Look, how people pursuing even after their sick sheep!
Now it would be unreasonable to expect anything but what has been reported about brutal sheep culling from a nation who kills their own child only for staring at a stranger.
@ Riaz Khan
we must come out of this self hating state of mind...She is doing this coz Australia is largest exporter of sheep to the world and this news has effected alot of business in her country and now they are pointing fingers on Pakistan for all the wrong doing....Raja must make sure that his voice goes international that Australians sent us sick sheep and now they are trying to deviate everyone's attention by pointing fingers on killing method.
Australian agriculture ministry statement worse than their PM - agricultural ministry sick Australian sheep came from your end, it's Pakistani health ministry to decide these sheep are sick or not, it's not your bleedy business to teach Pakistani ministry how to test sick sheep.
"I explained to him that Australians are distressed to see these acts of cruelty and that I wanted the matter investigated".
One may inquire as to why Australia sent diseased sheep to Pakistan? What we were supposed to do them, raise them as pets?
Old habits die hard, PM should have told her that, unlike Australians, we are no longer her Majesty's colonial subjects. If she didn't like the treatment then she can take the sheep back to Australia, in her official plane.
Wish our leaders cared this much about their fellow Pakistani human beings.
Now where are those TV anchors who were giving comments on sheep health like a veterinary expert?
Again we did everything possible to show a hard face to international community instead of soft face!