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The 35-year-old, who will undergo an examination of the problem on Tuesday, was unable to complete his 11th over for Hampshire against Nottinghamshire at Southampton and was forced to leave the field.
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Steyn has had a horrible run of injuries over the last two years, hampering his bid to become South Africa's record Test wicket-taker, though he did equal the record in Sri Lanka last month. Steyn and Shaun Pollock have each taken 421 Test wickets.
A serious shoulder injury kept him out for most of last year before a heel problem sustained in his comeback Test against India in February sidelined him once more.
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