The Democratic governors of Colorado and New York and the Republican governors of Maryland and Massachusetts all said they would not send members of their state's National Guard units for border duty.
"We will not be complicit in this ongoing human tragedy," New York Governor Andrew Cuomo, a Democrat, said on Tuesday.
"In the face of the federal government's inhumane treatment of immigrant families, New York will not deploy National Guard to the border," Cuomo said on Twitter.
US 'will not be a migrant camp': Trump
Larry Hogan, the Republican governor of Maryland, said he will not send any National Guard resources to the border "until this policy of separating children from their families has been rescinded."
Hogan said he had ordered a four-member helicopter crew which had been stationed in the border state of New Mexico to "immediately return" home.
"Immigration enforcement efforts should focus on criminals, not separating innocent children from their families," Hogan said.
John Hickenlooper, the Democratic governor of Colorado, signed an executive order on Monday that forbids the use of state resources "for the purpose of separating any child from his or her parent or legal guardian," a practice he called "cruel and un-American."
Massachusetts Governor Charlie Baker, a Republican, said the policy was "cruel and inhumane," and a National Guard helicopter crew which had been scheduled to go to the border later this month would not be deployed.
"They're not going to the border," Baker told reporters.
The Trump administration is facing condemnation at home and abroad for the separations, the product of a "zero-tolerance" policy on undocumented migrants.
Why is the Trump administration separating immigrant families?
US officials say more than 2,300 children have been separated from their parents or guardians since early May, when the "zero-tolerance" policy was announced.
President Donald Trump has accused Democrats of causing the crisis by blocking immigration reform legislation.
"If you don't have Borders, you don't have a Country!" the Republican president tweeted on Tuesday.
If you don’t have Borders, you don’t have a Country!
— Donald J. Trump (@realDonaldTrump) June 19, 2018
Trump announced plans in April to send thousands of National Guard troops to the border, where they could remain until his promised wall is constructed.
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