Nagako Tomioka, 58, chief priestess of Tomioka Hachimangu shrine in Tokyo, was attacked by Shigenaga Tomioka, 56, as she got out of a car on Thursday evening. Media reports said he was her younger brother.
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Also, a woman aged in her 30s who was with Shigenaga Tomioka attacked and stabbed the driver of the car with a Japanese sword, police said. The injuries to his shoulder and chest were not life-threatening.
After slashing the priestess, Shigenaga Tomioka then stabbed the younger woman to death with a sword, before killing himself, police said. The woman's identity was not given.
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Police declined to comment on the motive for the killings, but domestic media said the incident appeared to stem from a family feud. Shigenaga sent a threatening letter to his sister in 2006, saying he would "send her to hell", the Sankei newspaper said.
Japan has very restrictive gun laws and gun-related killings are rare. Shinto is the traditional religion of Japan and many shrines dot the country. The Tomioka Hachimangu shrine, established in 1927, has a close link with sumo and the emperor and empress visited in 2012.
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