Palestinian Prisoners Club chief Qaddura Fares said an agreement had been reached between the strikers and Israeli authorities on improving their conditions.
An Israel Prisons Service spokeswoman confirmed the hunger strike was over.
UN alarmed at conditions for Palestinian hunger-strikers
Israeli authorities conceded to one of the prisoners' main demands - that they should have two family visits a month instead of the one they were entitled to before the strike, the spokeswoman said.
The resolution of the strike came hours before the start of the Muslim holy month of Ramazan.
A number of the strikers had been in sharply declining health.
Palestinian president Mahmud Abbas had urged US counterpart Donald Trump to raise the issue with Israeli Prime Minister Benjamin Netanyahu during his visit to the region earlier this week.
Demonstrations in support of the prisoners had been held across the occupied West Bank leading to repeated bloody clashes with Israeli security forces.
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