Fast-bowler Ishant Sharma will also miss the second Test at the Eden Gardens in Kolkata after failing to recover from the mosquito-borne viral disease Chikungunya, which also forced him to sit out of the Kanpur opener.
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Uncapped Haryana off-spinner Jayant Yadav will replace Sharma for the second Test of the series, which India lead 1-0.
Rahul previously scored two hundreds and a fifty in four innings against the West Indies in Tests and a T20I recently. He made 32 and 38 in Kanpur and injured his right hamstring while running between the wickets.
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Gambhir, 34, has played 56 Tests for India, the last of them in England over two years ago. The left-handed opener, who has scored nine Test hundreds, was recalled to the Indian squad on the back of his strong form in domestic tournaments.
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